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I think each character being introduced in the order of their appearance as they were provided a beautiful transition of Prometheus story unfolding to the reader. we see the anger of Power against Prometheus and the compassion and sorrow toward Prometheus from Hephaestus. We see Chrous need for understanding. We see Ocean wanting Prometheus' confession of wrongdoing against Zeus. We see Hermes the messenger persuasion fall on deaf ears. Yet IO introduction I found most profound. She enters in a state of confusion in contrast to Prometheus he has complete clarity. She is sent wondering and being chased throughout the barren land for who knows how long, again in contrast Prometheus's punishment is to be still chained to a rock. Metaphorically Prometheus is being tied or bound to the earth, for which his crimes are connected and IO is left to wonder in confusion as though she is trapped in a dream-like state. Her wondering is going to free him from his still torment. Their connection is the most powerful depiction throughout the entire story.